A PCR-Based Method for Distinguishing between Two Common Beehive Bacteria, Paenibacillus larvae and Brevibacillus laterosporus
نویسندگان
چکیده
منابع مشابه
A PCR detection method for rapid identification of Paenibacillus larvae.
American foulbrood is a disease of larval honeybees (Apis mellifera) caused by the bacterium Paenibacillus larvae. Over the years attempts have been made to develop a selective medium for the detection of P. larvae spores from honey samples. The most successful of these is a semiselective medium containing nalidixic acid and pipermedic acid. Although this medium allows the growth of P. larvae a...
متن کاملDetection of Paenibacillus larvae larvae spores in honey and diseased larvae Samples by culture and PCR
American foulbrood (AFB) is the most serious brood disease of the honey bee. Traditional methods are reliable but rather slow simply because they are based on biochemical, morphological and physiological identification of cultivated isolates. The aim of this study is the detection of Paenibacillus larvae larvae spores in honey and diseased larvae samples by culture and PCR. Therefore 54 samp...
متن کاملPeBL1, a novel protein elicitor from Brevibacillus laterosporus
21 In our study, we report the identification, characterization, and gene cloning of a novel 22 protein elicitor (PeBL1) secreted from Brevibacillus laterosporus strain A60. Through a 23 purification process consisting of ion exchange chromatography and HPLC, we isolated a 24 protein that was identified by ESI-QTOF-MS/MS. The 351-bp PeBL1 gene produces a 25 12833 Da protein with 116 amino acids...
متن کاملCorrection for Sheflo et al., Complete Genome Sequences of Five Brevibacillus laterosporus Bacteriophages
Volume 1, no. 6, e00668-13, 2013. The species infected by the five bacteriophages was misidentified as Paenibacillus larvae. The article title should read as given above, and the organism identified as Paenibacillus larvae should be identified as Brevibacillus laterosporus throughout. Page 1: The abstract should read as follows. “Brevibacillus laterosporus is a secondary invader following Europ...
متن کاملGenome Sequences of Five Additional Brevibacillus laterosporus Bacteriophages
Brevibacillus laterosporus has been isolated from many different environments, including beehives, and produces compounds that are toxic to many organisms. Five B. laterosporus phages have been isolated previously. Here, we announce five additional phages that infect this bacterium, including the first B. laterosporus siphoviruses to be discovered.
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2018
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.01886-18